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Cell Based Assay Kits
Cell Based Assay [ELISA]
  • How do you get the antibody out of the vial?
  • There is no color development. Why is that?
  • What Cell types has this assay been utilized for in Celprogen's lab?
 
  1. How do you get the antibody out of the vial?
  • Do a quick spin of the vial.
  • For 1 plate, add 100µl of wash buffer into the vial and pipette up and down several times to mix.
  • Pipette out 50µl of the mixture and add to 5mLs of Wash Buffer.
  • Freeze the other 50µl of antibody/Wash Buffer mix until ready to use.
  • For 2 plates, add 100µl of wash buffer into the vial and pipette up and down several times to mix.
  • Remove all 100µl of Antibody/Wash Buffer mix and add to 10mLs of Wash Buffer.
  1. There is no color development. Why is that?
  • Make sure you added Substrate MN1 Concentrate (red cap) to the Substrate MN1 Diluent (Bottle A).
  • For 1 plate, use 64µl of Substrate MN1 Concentrate (red cap) and add to 5mLs of MN1
          Substrate Diluent (Bottle A) in a 15mL conical.
  • Mix gently and add 50µl of Substrate mix into each well.
  • For 2 plates, use 128µl of Substrate MN1 Concentrate (red cap) and add to 10 mLs of MN1
          Substrate Diluent (Bottle A) in a 15mL conical.
  • Read at 20 minutes. A pink color should develop within 20 minutes.
  • Check cell density by viewing plate under a microscope.
  • If reagents are stored at 2-8˚C make sure they have reached room temperature before using.
  • Make sure you have utilized HRP secondary antibody after the primary antibody incubation.
  • Make sure you have utilized the substrate after the secondary antibody incubation.
  • Make sure you have not cross contaminated reagents.

                  NOTE: Check cell density in between washes. Assay is very sensitive and may remove cells
                              if not preformed gently. Before beginning assay, make sure there is a high cell density. 

  1. What cells types has this assay been utilized for in Celprogen's lab?
  • Human Embryonic Stem Cells and Human Mesenchymal Stem Cells

                  NOTE: This kit has been optimized for the provided reagents and only works with these reagents.