Human Normal Microglia Tissue cDNA: 50ug

 

Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 110 ng total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40 µl final volume. RT Reaction stopped by heating at 70°C for 10 minutes. The cDNA is in 1x RT buffer. (1x RT Buffer: 50 mM Tris-Cl, pH 8.3, 75 mM KCI, 3 mM MgCl2, 10mM DTT). The estimated cDNA concentration is about 2.5 ng/µl. 1 µl cDNA is good enough for one PCR reaction.

 

Source: Adult Human Normal Microglia Tissue

 

Storage Conditions: -20°C or colder