Cell Based ELISA Kit
This product is also avaiable with Human Breast Cancer Stem Cell Frozen Vial Cells Cat# 36102-29
This product is also available with Human Breast Cancer Stem Cell T25 Plated Cells Cat# 36102-29-T25
This product is also avaiable with Human Breast Cancer Stem Cell Media Cat# M36102-29
This Cell-Based ELISA Kit contains components required to measure one protein in whole cells to determine the stem cell's differentiation or un-differentiation status.
Utilizing this Cell Based assay format, Breast Cancer Stem Cell stem cell's differentiation or un-differentiation fate can be determined. This simple and efficient assay eliminates the need to prepare cell lysates and is broadly applicable for drug discovery and drug screening.
This assay format may be used to assess stem cell differentiation status or effect of stimulation and/or inhibition of compounds and ligands on target molecules directly associated with stem cell differentiation or maintenance of stem cells in their un-differentiation stage.
Cells are grown in 96-well plates pre-coated with extracellular matrix and may be stimulated with ligands and/or incubated with inhibitors. Cells are then fixed and permeabilized in the wells. The status of target protein in response to treatment is analyzed using an immunoenzymatic labeling procedure.
The cells are incubated with one primary antibody: A rabbit or mouse monoclonal antibody specific for the protein of interest.
After washing away unbound antibodies,anti-rabbit and or anti-mouse secondary antibodies labeled with horseradish-peroxidase (HRP) or alkaline phosphatase (AP), respectively, and two spectrally distinct fluorogenic substrates for either HRP or AP may be used for detection.